U Uji Aktivitas Antibakteri Ekstrak Daun Pulutan (Urena lobata L) Terhadap Bakteri Streptococcus pyogenes

Shela Pusvita; Rohama Rohama; Iwan Yuwindry; Putri Vidiasari Darsono

doi:10.33859/jpcs.v4i1.420

Authors

Shela Pusvita a:1:{s:5:"en_US";s:21:"University Sari Mulia";}
Rohama Rohama Sari Mulia University
Iwan Yuwindry Sari Mulia University
Putri Vidiasari Darsono Sari Mulia University

DOI:

https://doi.org/10.33859/jpcs.v4i1.420

Keywords:

Streptococcus pyogenes., Pulutan Leaf Extract, Antibacterial

Abstract

Background: Pharyngitis or sore throat has a fairly high prevalence rate in Indonesia caused by Streptococcus pyogenes bacteria. Pulutan has experience as a medicine for sore throat, rheumatic fever, antipyretic, fracture, antifertility, and vaginal discharge. Based on research on ethanol extract that was done previously, pulutan leaf extract has antibacterial activity.

Objective: To determine the antibacterial activity of pulutan leaf extract (Urena lobata L) which has the potential as an antibacterial against the growth of Streptococcus pyogenes and to determine the minimum inhibitory concentration and minimum killing concentration.

Methods: This type of true experimental research design, namely research methods that look for causal relationships between independent and dependent variables. Antibacterial testing using disc diffusion and dilution methods. With concentrations of 100%, 75%, 50%, 25%, positive control amoxicillin and DMSO negative control.

Results: The results of the disc diffusion test were able to inhibit the growth of the Streptococcus pyogenes bacteria which was indicated by the presence of a clear zone around the disc paper. The results of the dilution test obtained the MIC value at a concentration of 50% indicated by the media tube which looks clear. There was no KBM value at all the concentrations of the extracts tested, indicated by the growth of bacteria in the test medium.

Conclusion: Pulutan leaf extract (Urena lobata L) has antibacterial activity which is indicated by the presence of an inhibition/clear zone around the disc paper. MIC results found at a concentration of 50%, KBM results on pulutan leaf extract (Urena lobata L) did not have killing power at all concentrations.

Keywords: Antibacterial, Pulutan Leaf Extract (Urena lobata L), Streptococcus pyogenes.

References

Andryanie, Y. (2020). Uji Aktivitas Antibakteri Ekstrak Etanol Batang Dadangkak (Hydrolea spinosa) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli.

Desai, N., Steenbergen, J., & Katz, D. E. (2017). Antibiotic Resistance of Non-pneumococcal Streptococci and Its Clinical Impact. Antimicrobial Drug Resistance, 791–810. https://doi.org/10.1007/978-3-319-47266-9_2

Khasanah, Y. C. (2018). Potensi Koekspresi Chimeric Antigen Receptor (Car) Dan Gen Foxp3 Pada Sel T Regulators Sebagai Modalitas Terapi Penatalaksanaan Autoimun. Essence of Scientific Medical Journal, 16(2), 26–30.

Lestari, D. L. P. A., Jayanti, N. P. S. D., Putra, T. W., Fridayanthi, P. U., Tjahyadi, I. G. K. D. P. P., Maharani, L. G. S., & Cahyawati, P. N. (2022). Diagnosis Dan Tatalaksana Faringitis Streptococcus Group a. WICAKSANA: Jurnal Lingkungan Dan Pembangunan, 6(2), 88–95. https://doi.org/10.22225/wicaksana.6.2.2022.88-95

Santi, H. M. (2020). Aktivitas Antibakteri dan Profil Bioautografi Fraksi Etil Asetat Daun Pulutan (Urena lobota L) Terhadap Bakteri Staphylococcus aureus. Analisis Standar Pelayanan Minimal Pada Instalasi Rawat Jalan Di RSUD Kota Semarang, 3, 103–111.